Targeting duplex DNA with DNA-PNA chimeras? Physico-chemical characterization of a triplex DNA-PNA/DNA/DNA.

Authors :: Petraccone L
Erra E
Messere A
Montesarchio D
Piccialli G
De Napoli L
Barone G
Giancola C
Source :: Biopolymers [Biopolymers] 2004 Mar; Vol. 73 (4), pp. 434-42.
Publication Year :: 2004
Abstract: Targeting double-stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA-PNA chimeras. A 16-mer 5'-DNA-3'-p-(N)PNA(C) has been investigated for its ability to hybridize a complementary duplex DNA by DSC, CD, and molecular modeling studies. The obtained results showed the formation of a triplex structure having similar, if not slightly higher, stability compared to the same all-DNA complex.<br /> (Copyright 2004 Wiley Periodicals, Inc.)

Subjects :: Chemical Phenomena
Chemistry, Physical
Circular Dichroism
DNA chemical synthesis
DNA metabolism
Hydrogen Bonding
Hydrogen-Ion Concentration
Models, Molecular
Molecular Structure
Oligodeoxyribonucleotides chemical synthesis
Peptide Nucleic Acids chemical synthesis
Peptide Nucleic Acids metabolism
Pyrimidine Nucleotides chemistry
Temperature
Thermodynamics
Chimera
DNA chemistry
Nucleic Acid Conformation
Peptide Nucleic Acids chemistry

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