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Occludin expression decreases with the progression of human endometrial carcinoma.
- Source :
-
Human pathology [Hum Pathol] 2004 Feb; Vol. 35 (2), pp. 159-64. - Publication Year :
- 2004
-
Abstract
- The tight junctions of the glandular epithelium are crucial for the maintenance of cell polarity, separating the plasma membrane into apical and basolateral domains. Thus abnormalities of the tight junctions may result in the structural disturbances of glandular epithelial neoplasia. In this study we introduced an anti-occludin monoclonal antibody for semiquantitative assay of the occludin expression in tissue sections of human normal and neoplastic endometrial epithelia using the Adobe Photoshop and NIH Image programs. Normal endometrial glands and samples of endometrial hyperplasia and endometrioid carcinoma grade 1 fully expressed occludin at the apical cell border. In endometrioid carcinomas grades 2 and 3, however, occludin disappeared in solid areas of the carcinomatous tissues. Occludin was also found at the apical borders of the cancer cells that formed glandular structures. Occludin expression decreased progressively in parallel with the increase in carcinoma grade, and the decreased occludin expression correlated with myometrial invasion and lymph node metastasis. These results suggest that the loss of tight junctions has a close relationship with structural atypia in the progression of human endometrial carcinomas and their malignant potential.
- Subjects :
- Adenocarcinoma metabolism
Adenocarcinoma pathology
Adult
Antibodies, Monoclonal
Biomarkers, Tumor immunology
Disease Progression
Down-Regulation
Endometrial Hyperplasia metabolism
Endometrial Hyperplasia pathology
Female
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Lymphatic Metastasis
Membrane Proteins immunology
Neoplasm Invasiveness
Occludin
Biomarkers, Tumor metabolism
Endometrial Neoplasms metabolism
Endometrial Neoplasms pathology
Membrane Proteins metabolism
Tight Junctions metabolism
Tight Junctions pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0046-8177
- Volume :
- 35
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Human pathology
- Publication Type :
- Academic Journal
- Accession number :
- 14991532
- Full Text :
- https://doi.org/10.1016/j.humpath.2003.09.013