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Lipocalin-type prostaglandin D synthase in urine in adriamycin-induced nephropathy of mice.
- Source :
-
Nephron. Physiology [Nephron Physiol] 2004; Vol. 96 (2), pp. p42-51. - Publication Year :
- 2004
-
Abstract
- Background/aims: Lipocalin-type prostaglandin D synthase (L-PGDS), an enzyme converting prostaglandin H(2) to prostaglandin D(2), occurs particularly in the cardiovascular system. Urinary L-PGDS excretion is increased in diabetes prior to overt proteinuria, suggesting that it is a predictor of renal injury. In this study, we tested the hypothesis that L-PGDS excretion reflects renal injury in primary glomerular diseases using Adriamycin-induced nephropathy in mice.<br />Methods: Twenty 6-week-old ICR female mice were intravenously given a dose of 25 mg Adriamycin/kg body weight through the tail vein. 24-hour urine was collected every day, and blood samples were obtained.<br />Results: The mice developed significant albuminuria from day 3 onward (p < 0.05), which was followed by overt proteinuria from day 4 (p < 0.05). Histological examination revealed focal mesangial expansion with partial tubular atrophy. Urinary L-PGDS excretion significantly increased from day 1 onward (p < 0.05), and apparently preceded the increase in urinary albumin excretions. Either serum L-PGDS or creatinine levels were not changed by administration of Adriamycin. However, serum creatinine levels were inversely correlated to urinary L-PGDS excretions (r = -0.88, p < 0.05). Immunohistochemistry showed that L-PGDS occurred in the tubules, but not in the glomeruli in Adriamycin mice and L-PGDS mRNA paralleled urinary L-PGDS excretion.<br />Conclusion: Urinary L-PGDS excretion is increased in Adriamycin-induced nephropathy, and this precedes overt albuminuria.<br /> (Copyright 2004 S. Karger AG, Basel)
- Subjects :
- Animals
Body Weight
Doxorubicin
Female
Glomerulonephritis chemically induced
Glomerulonephritis pathology
Intramolecular Oxidoreductases blood
Intramolecular Oxidoreductases metabolism
Kidney pathology
Kidney physiopathology
Lipocalins
Mice
Mice, Inbred ICR
Organ Size
RNA, Messenger metabolism
Glomerulonephritis urine
Intramolecular Oxidoreductases urine
Subjects
Details
- Language :
- English
- ISSN :
- 1660-2137
- Volume :
- 96
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Nephron. Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 14988661
- Full Text :
- https://doi.org/10.1159/000076407