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Expression of vanilloid receptor subtype 1 in cutaneous sensory nerve fibers, mast cells, and epithelial cells of appendage structures.
- Source :
-
Experimental dermatology [Exp Dermatol] 2004 Mar; Vol. 13 (3), pp. 129-39. - Publication Year :
- 2004
-
Abstract
- The vanilloid receptor subtype 1 (VR1)/(TRPV1), binding capsaicin, is a non-selective cation channel that recently has been shown in human keratinocytes in vitro and in vivo. However, a description of VR1 localization in other cutaneous compartments in particular cutaneous nerve fibers is still lacking. We therefore investigated VR1 immunoreactivity as well as mRNA and protein expression in a series (n = 26) of normal (n = 7), diseased (n = 13) [prurigo nodularis (PN) (n = 10), generalized pruritus (n = 1), and mastocytosis (n = 2)], and capsaicin-treated human skin (n = 6). VR1 immunoreactivity could be observed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands. Upon reverse transcriptase-polymerase chain reaction and Western blot analysis, VR1 was detected in mast cells and keratinocytes from human skin. In pruritic skin of PN, VR1 expression was highly increased in epidermal keratinocytes and nerve fibers, which was normalized after capsaicin application. During capsaicin therapy, a reduction of neuropeptides (substance P, calcitonin gene-related peptide) was observed. After cessation of capsaicin therapy, neuropeptides re-accumulated in skin nerves. In conclusion, VR1 is widely distributed in the skin, suggesting a major role for this receptor, e.g. in nociception and neurogenic inflammation.
- Subjects :
- Adolescent
Adult
Aged
Animals
Biopsy
Brain metabolism
Calcitonin Gene-Related Peptide metabolism
Capillaries metabolism
Capsaicin metabolism
Capsaicin therapeutic use
Cell Line
Child
Eccrine Glands metabolism
Epidermal Cells
Epidermis metabolism
Epithelial Cells cytology
Gene Expression
Hair Follicle metabolism
Humans
Mast Cells cytology
Middle Aged
Nerve Fibers metabolism
Neurons, Afferent ultrastructure
Nociceptors physiology
Pruritus drug therapy
Pruritus pathology
Rats
Receptors, Drug metabolism
Sebaceous Glands metabolism
Spinal Cord metabolism
Substance P metabolism
Epidermis innervation
Epithelial Cells physiology
Mast Cells physiology
Neurons, Afferent physiology
Pruritus physiopathology
Receptors, Drug genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0906-6705
- Volume :
- 13
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Experimental dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 14987252
- Full Text :
- https://doi.org/10.1111/j.0906-6705.2004.0178.x