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Singlet oxygen-mediated damage to cellular DNA determined by the comet assay associated with DNA repair enzymes.

Authors :
Ravanat JL
Sauvaigo S
Caillat S
Martinez GR
Medeiros MH
Di Mascio P
Favier A
Cadet J
Source :
Biological chemistry [Biol Chem] 2004 Jan; Vol. 385 (1), pp. 17-20.
Publication Year :
2004

Abstract

The damage profile produced by the reaction of singlet molecular oxygen with cellular DNA was determined using the comet assay associated with DNA repair enzymes. Singlet oxygen was produced intracellularly by thermal decomposition of a water-soluble endoperoxide of a naphthalene derivative which is able to penetrate through the membrane into mammalian cells. We found that the DNA modifications produced by singlet oxygen were almost exclusively oxidised purines recognised by the formamidopyrimidine DNA N-glycosylase. In contrast, significant amounts of direct strand breaks and alkali-labile sites or oxidised pyrimidines, detectable by the bacterial endonuclease III, were not produced.

Details

Language :
English
ISSN :
1431-6730
Volume :
385
Issue :
1
Database :
MEDLINE
Journal :
Biological chemistry
Publication Type :
Academic Journal
Accession number :
14977042
Full Text :
https://doi.org/10.1515/BC.2004.003