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Detection of equine herpesvirus 3 in equine skin lesions by polymerase chain reaction.
- Source :
-
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2004 Jan; Vol. 16 (1), pp. 74-9. - Publication Year :
- 2004
-
Abstract
- During a recent breeding season, ulcerative, pustular skin lesions were observed on the external genitalia of 2 mares and 1 stallion within a small herd. Based on the location and description of the skin lesions plus the clinical history, equine coital exanthema, caused by equine herpesvirus 3 (EHV3), was the primary differential diagnosis. Scrapings of skin lesions from the perineum of 2 mares were submitted for diagnostic evaluation. Virus isolation was attempted by inoculation of several cell lines of equine origin, but no cytopathic agent was detected. The skin scrapings were processed for DNA extraction, and polymerase chain reaction (PCR) amplification was performed for herpesvirus DNA polymerase and DNA-packaging protein (terminase) genes using nested, degenerate primers targeted to conserved regions of the herpesvirus genome. Products of the expected sizes were generated for both assays, and subsequent nucleotide sequencing of the amplification products established that EHV3 had been detected in DNA extracted from the skin lesions. Detection of EHV3 was confirmed using an EHV3-specific PCR assay targeted to the gC gene. Using the novel EHV3 nucleotide sequence identified in this report, a sensitive and specific PCR assay targeted to the highly conserved DNA polymerase gene was developed.
- Subjects :
- Animals
Base Sequence
DNA, Viral chemistry
DNA, Viral genetics
DNA-Directed DNA Polymerase chemistry
DNA-Directed DNA Polymerase genetics
Female
Genital Diseases, Female pathology
Genital Diseases, Female virology
Herpesviridae Infections pathology
Herpesviridae Infections virology
Herpesvirus 3, Equid genetics
Horse Diseases pathology
Horses
Male
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction veterinary
Sequence Alignment
Genital Diseases, Female veterinary
Herpesviridae Infections veterinary
Herpesvirus 3, Equid isolation & purification
Horse Diseases virology
Subjects
Details
- Language :
- English
- ISSN :
- 1040-6387
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Publication Type :
- Academic Journal
- Accession number :
- 14974851
- Full Text :
- https://doi.org/10.1177/104063870401600113