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Detection and nucleotide sequencing of a DNA-packaging protein gene of equine gammaherpesviruses.
- Source :
-
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2004 Jan; Vol. 16 (1), pp. 67-74. - Publication Year :
- 2004
-
Abstract
- In previous studies, novel putative viral pathogens designated that asinine herpesvirus 4 (AsHV4) and asinine herpesvirus 5 (AsHV5) were associated with fatal interstitial pneumonia in donkeys (Equus asinus). Nucleotide sequence analysis of a portion of the DNA polymerase gene identified these putative pathogens as herpesviruses and possibly as members of the Gammaherpesvirinae subfamily. Although similar to equine herpesvirus 2 (EHV2) and equine herpesvirus 5 (EHV5), sequence diversity was observed among the detected viruses. In this study, novel sequence is reported for a DNA-packaging protein gene of EHV5 plus AsHV4, AsHV5, and a newly described putative pathogen herein designated asinine herpesvirus 6 (AsHV6). Phylogenetic analysis of these sequences suggested that the equine gammaherpesviruses may form a separate clade within the Gammaherpesvirinae subfamily. Based on the sequence of EHV2 and the novel sequences reported in this study, a PCR assay was developed to detect equine gammaherpesviruses. Products of the predicted size were produced after amplification of DNA from EHV2, EHV5, AsHV4, AsHV5, and AsHV6. This nonnested assay was shown to consistently amplify approximately 10 genomic copies of EHV2. Amplification products were not produced from DNA template of other alpha- and gammaherpesviruses. Because the role of gammaherpesviruses has not been well defined in equine disease, it is envisioned that a single, sensitive PCR assay to detect these potential pathogens will facilitate further assessment of their role in disease.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
DNA, Viral chemistry
DNA, Viral genetics
DNA-Directed DNA Polymerase chemistry
DNA-Directed DNA Polymerase genetics
Gammaherpesvirinae classification
Gammaherpesvirinae enzymology
Herpesviridae Infections virology
Horses
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction veterinary
Sequence Alignment
Endodeoxyribonucleases genetics
Gammaherpesvirinae genetics
Herpesviridae Infections veterinary
Horse Diseases virology
Subjects
Details
- Language :
- English
- ISSN :
- 1040-6387
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Publication Type :
- Academic Journal
- Accession number :
- 14974850
- Full Text :
- https://doi.org/10.1177/104063870401600112