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Characterization of elongated Helicobacter pylori isolated from a patient with gastric-mucosa-associated lymphoid-tissue lymphoma.
- Source :
-
Journal of medical microbiology [J Med Microbiol] 2004 Mar; Vol. 53 (Pt 3), pp. 207-212. - Publication Year :
- 2004
-
Abstract
- To date, two Helicobacter species, Helicobacter pylori and 'Helicobacter heilmannii' (formerly named 'Gastrospirillum hominis'), have been identified from the human stomach. In this study, we observed non-H. pylori-shaped bacteria in gastric tissue sections and successfully isolated them by cultivation. Elongated bacteria were isolated from a patient with gastric-mucosa-associated lymphoid-tissue lymphoma who had been diagnosed as H. pylori-negative by culture, rapid urease test and histopathology in another hospital. The bacteria were grown only on chocolate agar in a CO2 incubator, appeared more than 10 microm long in histological sections, formed small colonies and showed poor growth in a brain heart infusion broth; these characteristics apparently differed from common clinical isolates of H. pylori. However, the bacteria were identified as H. pylori by PCR of the urease gene, 16S rDNA sequencing, protein profile and antigenicity examined by anti-H. pylori polyclonal antibody. These observations suggest that the H. pylori strain identified in this study may contribute to the development of gastroduodenal diseases in cases judged as H. pylori-negative by ordinary methods.
- Subjects :
- Bacterial Proteins analysis
Bacterial Proteins immunology
Blotting, Western
DNA, Bacterial analysis
DNA, Ribosomal analysis
Gastric Mucosa pathology
Helicobacter pylori classification
Helicobacter pylori genetics
Helicobacter pylori ultrastructure
Humans
Microscopy, Electron
Microscopy, Electron, Scanning
Polymerase Chain Reaction
RNA, Ribosomal, 16S genetics
Urease genetics
Gastric Mucosa microbiology
Helicobacter pylori isolation & purification
Lymphoma, B-Cell, Marginal Zone microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-2615
- Volume :
- 53
- Issue :
- Pt 3
- Database :
- MEDLINE
- Journal :
- Journal of medical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 14970245
- Full Text :
- https://doi.org/10.1099/jmm.0.05281-0