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Defects in translational regulation mediated by the alpha subunit of eukaryotic initiation factor 2 inhibit antiviral activity and facilitate the malignant transformation of human fibroblasts.
- Source :
-
Molecular and cellular biology [Mol Cell Biol] 2004 Mar; Vol. 24 (5), pp. 2025-40. - Publication Year :
- 2004
-
Abstract
- Suppression of protein synthesis through phosphorylation of the translation initiation factor alpha subunit of eukaryotic initiation factor 2 (eIF2alpha) is known to occur in response to many forms of cellular stress. To further study this, we have developed novel cell lines that inducibly express FLAG-tagged versions of either the phosphomimetic eIF2alpha variant, eIF2alpha-S51D, or the phosphorylation-insensitive eIF2alpha-S51A. These variants showed authentic subcellular localization, were incorporated into endogenous ternary complexes, and were able to modulate overall rates of protein synthesis as well as influence cell division. However, phosphorylation of eIF2alpha failed to induce cell death or sensitize cells to killing by proapoptotic stimuli, though it was able to inhibit viral replication, confirming the role of eIF2alpha in host defense. Further, although the eIF2alpha-S51A variant has been shown to transform NIH 3T3 cells, it was unable to transform the murine fibroblast 3T3 L1 cell line. To therefore clarify this issue, we explored the role of eIF2alpha in growth control and demonstrated that the eIF2alpha-S51A variant is capable of collaborating with hTERT and the simian virus 40 large T antigen in the transformation of primary human kidney cells. Thus, dysregulation of translation initiation is indeed sufficient to cooperate with defined oncogenic elements and participate in the tumorigenesis of human tissue.
- Subjects :
- Animals
Apoptosis physiology
Cell Size
Cells, Cultured
DNA-Binding Proteins
Eukaryotic Initiation Factor-2 genetics
Fibroblasts cytology
Genes, Reporter
Humans
Macromolecular Substances
Mice
NIH 3T3 Cells
Phosphorylation
Protein Subunits genetics
Telomerase genetics
Telomerase metabolism
Cell Transformation, Neoplastic
Eukaryotic Initiation Factor-2 metabolism
Fibroblasts physiology
Gene Expression Regulation
Protein Biosynthesis
Protein Subunits metabolism
Virus Replication physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0270-7306
- Volume :
- 24
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Molecular and cellular biology
- Publication Type :
- Academic Journal
- Accession number :
- 14966282
- Full Text :
- https://doi.org/10.1128/MCB.24.5.2025-2040.2004