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Improved flow cytometric method for HLA-B27 typing.
- Source :
-
Annals of clinical biochemistry [Ann Clin Biochem] 1992 Nov; Vol. 29 ( Pt 6), pp. 663-7. - Publication Year :
- 1992
-
Abstract
- HLA-B27 is a cell marker of clinical interest because of its high association with certain diseases. The HLA-B27 antigen was detected on lymphocytes using a monoclonal antibody in an indirect immuno-fluorescence assay using a fluorescence flow cytometer. The considerable crossreaction of the monoclonal antibody with the HLA-B7 antigen was effectively suppressed by masking it by means of human anti-HLA-B7 antiserum. The flow cytometric method was evaluated by comparing the results with those obtained by the standard lymphocytotoxicity test and showed complete agreement in 107 selected patient samples.
Details
- Language :
- English
- ISSN :
- 0004-5632
- Volume :
- 29 ( Pt 6)
- Database :
- MEDLINE
- Journal :
- Annals of clinical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 1489163
- Full Text :
- https://doi.org/10.1177/000456329202900608