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Purification, characterization, and gene cloning of cis,cis-muconate cycloisomerase from benzamide-assimilating Arthrobacter sp. BA-5-17.
- Source :
-
FEMS microbiology letters [FEMS Microbiol Lett] 2004 Feb 09; Vol. 231 (1), pp. 119-24. - Publication Year :
- 2004
-
Abstract
- cis,cis-Muconate cycloisomerase (MC) was purified to homogeneity from benzamide-assimilating Arthrobacter sp. BA-5-17. The purified enzyme showed high activities for cis,cis-muconate and 3-methyl-cis,cis-muconate, and preferred the 3-substituted derivatives over the derivatives with the same substituent at the 2 position as a substrate. A gene encoding MC of strain BA-5-17 was cloned and named catB. The catB gene was clustered with catR encoding a putative LysR-type regulator, catC encoding a putative muconolactone isomerase, and catA-II encoding the catechol 1,2-dioxygenase isozymes CD-III-1 and III-2. These genes showed the same orientation in transcriptional direction and the organization of cloned genes was catRBCA-II. In the phylogenetic analysis of MCs and chloro-MCs, the BA-5-17 and Streptomyces setonii MCs formed a subfamily, clearly distinguished from those of other MCs.
- Subjects :
- Amino Acid Sequence
Arthrobacter enzymology
Arthrobacter metabolism
Genes, Regulator
Intramolecular Lyases isolation & purification
Intramolecular Lyases metabolism
Molecular Sequence Data
Phylogeny
Arthrobacter genetics
Benzamides metabolism
Catechols metabolism
Genes, Bacterial
Intramolecular Lyases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1097
- Volume :
- 231
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEMS microbiology letters
- Publication Type :
- Academic Journal
- Accession number :
- 14769475
- Full Text :
- https://doi.org/10.1016/S0378-1097(03)00933-9