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High-level accumulation of a recombinant antibody fragment in the periplasm of Escherichia coli requires a triple-mutant (degP prc spr) host strain.
- Source :
-
Biotechnology and bioengineering [Biotechnol Bioeng] 2004 Mar 05; Vol. 85 (5), pp. 463-74. - Publication Year :
- 2004
-
Abstract
- During production of a humanized antibody fragment secreted into the periplasm of Escherichia coli, proteolytic degradation of the light chain was observed. In order to determine which protease(s) were responsible for this degradation, we compared expression of the F(ab')(2) antibody fragment in several E. coli strains carrying mutations in genes encoding periplasmic proteases. Analysis of strains cultured in high cell density fermentations showed that the combination of mutations in degP prc spr was necessary for the cells to produce high levels of the desired recombinant antibody fragment. In order to eliminate the possible effects of mutations in other genes, we constructed E. coli strains with protease mutations in isogenic backgrounds and repeated the studies in high cell density fermentations. Extensive light chain proteolysis persisted in degP strains. However, light chain proteolysis was substantially decreased in prc and prc spr strains, and was further decreased with the introduction of a degP mutation in prc and prc spr mutant strains. These results show that the periplasmic protease Prc (Tsp) is primarily responsible for proteolytic degradation of the light chain during expression of a recombinant antibody fragment in E. coli, and that DegP (HtrA) makes a minor contribution to this degradation as well. The results also show that spr, a suppressor of growth defects in prc strains, is required for a prc mutant to survive throughout high cell density fermentations.<br /> (Copyright 2004 Wiley Periodicals, Inc.)
- Subjects :
- CD18 Antigens immunology
Cell Division
Cell Survival
Endopeptidases genetics
Escherichia coli cytology
Escherichia coli growth & development
Gene Expression Regulation, Bacterial physiology
Heat-Shock Proteins genetics
Heat-Shock Proteins metabolism
Immunoglobulin Fragments chemistry
Immunoglobulin Fragments immunology
Mutagenesis, Site-Directed
Periplasm metabolism
Periplasmic Proteins genetics
Periplasmic Proteins metabolism
Recombinant Proteins biosynthesis
Serine Endopeptidases genetics
Serine Endopeptidases metabolism
Species Specificity
Cell Culture Techniques methods
Endopeptidases metabolism
Escherichia coli genetics
Escherichia coli metabolism
Immunoglobulin Fragments biosynthesis
Immunoglobulin Fragments genetics
Protein Engineering methods
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3592
- Volume :
- 85
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Biotechnology and bioengineering
- Publication Type :
- Academic Journal
- Accession number :
- 14760686
- Full Text :
- https://doi.org/10.1002/bit.20014