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Use of expressed sequence tag analysis and cDNA microarrays of the filamentous fungus Aspergillus nidulans.
- Source :
-
Fungal genetics and biology : FG & B [Fungal Genet Biol] 2004 Feb; Vol. 41 (2), pp. 199-212. - Publication Year :
- 2004
-
Abstract
- The use of microarrays in the analysis of gene expression is becoming widespread for many organisms, including yeast. However, although the genomes of a number of filamentous fungi have been fully or partially sequenced, microarray analysis is still in its infancy in these organisms. Here, we describe the construction and validation of microarrays for the fungus Aspergillus nidulans using PCR products from a 4092 EST conidial germination library. An experiment was designed to validate these arrays by monitoring the expression profiles of known genes following the addition of 1% (w/v) glucose to wild-type A. nidulans cultures grown to mid-exponential phase in Vogel's minimal medium with ethanol as the sole carbon source. The profiles of genes showing statistically significant differential expression following the glucose up-shift are presented and an assessment of the quality and reproducibility of the A. nidulans arrays discussed.
- Subjects :
- Aspergillus nidulans metabolism
Culture Media chemistry
DNA, Fungal isolation & purification
Ethanol metabolism
Fungal Proteins genetics
Fungal Proteins physiology
Gene Expression Regulation, Fungal
Gene Library
Genes, Fungal
Gluconeogenesis genetics
Glyoxylates metabolism
Reproducibility of Results
Aspergillus nidulans genetics
Aspergillus nidulans growth & development
Expressed Sequence Tags
Gene Expression Profiling methods
Glucose metabolism
Oligonucleotide Array Sequence Analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1087-1845
- Volume :
- 41
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Fungal genetics and biology : FG & B
- Publication Type :
- Academic Journal
- Accession number :
- 14732266
- Full Text :
- https://doi.org/10.1016/j.fgb.2003.11.005