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A fluorescent intercalator displacement assay for establishing DNA binding selectivity and affinity.
- Source :
-
Accounts of chemical research [Acc Chem Res] 2004 Jan; Vol. 37 (1), pp. 61-9. - Publication Year :
- 2004
-
Abstract
- A summary of the qualitative and quantitative elements of a fluorescent intercalator displacement (FID) assay useful for establishing the DNA binding selectivity, affinity, stoichiometry, and binding site size and distinguishing modes of DNA binding is provided.
- Subjects :
- Base Sequence
Binding, Competitive
DNA metabolism
Fluorescent Dyes metabolism
Netropsin chemistry
Netropsin metabolism
Nylons chemistry
Nylons metabolism
Oligodeoxyribonucleotides chemistry
Oligodeoxyribonucleotides metabolism
Thiazoles chemistry
Thiazoles metabolism
DNA chemistry
Fluorescent Dyes chemistry
Spectrometry, Fluorescence methods
Subjects
Details
- Language :
- English
- ISSN :
- 0001-4842
- Volume :
- 37
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Accounts of chemical research
- Publication Type :
- Academic Journal
- Accession number :
- 14730995
- Full Text :
- https://doi.org/10.1021/ar030113y