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Inhibition of human cytomegalovirus replication by small interfering RNAs.
- Source :
-
The Journal of general virology [J Gen Virol] 2004 Jan; Vol. 85 (Pt 1), pp. 179-184. - Publication Year :
- 2004
-
Abstract
- Small interfering RNAs (siRNAs) are the mediators of a sequence-specific process of gene silencing called RNA interference (RNAi). Here, we show that synthetic siRNAs against essential gene products of human cytomegalovirus (HCMV) can trigger RNAi in serum-starved, infected primary fibroblasts, as well as in U373 cells, leading to effective inhibition of viral DNA replication. This opens new possibilities for antiviral strategies and for the analysis of viral and cellular genes important to HCMV physiology.
- Subjects :
- Cell Line
Cells, Cultured
DNA-Directed DNA Polymerase genetics
DNA-Directed DNA Polymerase metabolism
Down-Regulation
Fibroblasts virology
Gene Expression Regulation, Viral
Humans
RNA, Small Interfering metabolism
Transfection
Cytomegalovirus physiology
RNA Interference
RNA, Small Interfering pharmacology
Viral Proteins
Virus Replication drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1317
- Volume :
- 85
- Issue :
- Pt 1
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 14718633
- Full Text :
- https://doi.org/10.1099/vir.0.19453-0