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Evaluation of the nitric oxide production in rat renal artery smooth muscle cells culture exposed to radiocontrast agents.
- Source :
-
Kidney international [Kidney Int] 2004 Feb; Vol. 65 (2), pp. 589-96. - Publication Year :
- 2004
-
Abstract
- Background: Radiocontrast agents (RC), substances largely used in diagnostic procedures, present the nephrotoxicity as one of its major side effects, which could be due to an altered synthesis of vasodilators. The aim of the present study was to evaluate the nitric oxide (NO) production in rat renal artery smooth muscle cells primary culture (rVSMC) exposed to RC.<br />Methods: The cells were treated for 72 hours with mannitol at 10% (MT10; 600 mOsm/kg H2O) or 35% (MT35; 2100 mOsm/kg H2O), with the nonionic iobitridol (IBT), the low-osmolality ioxaglate (IXG), the high-osmolality ioxitalamate (IXT), the nonionic, iso-osmolar iodixanol (IDX), and with lipopolysaccharide (LPS). We determined the NO and osmolality in the cell culture media and the cellular viability.<br />Results: By the Griess and chemiluminescence methods, the NO was not different in MT10 and IDX, but decreased in MT35, IBT, IXG, and IXT when compared with the control; it was increased in LPS and also decreased in all RC+LPS when compared with LPS. MT35, IXT, and IXT+LPS decreased the cellular viability, and the media osmolality was increased in MT35 and IXT compared with the control.<br />Conclusion: The RC (except IDX) significantly reduced NO in rVSMC, which was more pronounced after IXT treatment (57.3%). This was not related to the reduced cell viability (15.8%) or to its high osmolality, because in MT35, with similar osmolality as IXT, NO decreased only 11.0% relatively to the control. Neither the media osmolality nor the cell viability was altered by IXG or IBT. The decreased NO could explain the vasoconstriction and, therefore, the acute renal failure by RC.
- Subjects :
- Animals
Cells, Cultured
Dose-Response Relationship, Drug
Iothalamic Acid pharmacology
Ioxaglic Acid pharmacology
Male
Muscle, Smooth, Vascular cytology
Muscle, Smooth, Vascular metabolism
Rats
Rats, Wistar
Triiodobenzoic Acids pharmacology
Contrast Media pharmacology
Iohexol analogs & derivatives
Iohexol pharmacology
Iothalamic Acid analogs & derivatives
Muscle, Smooth, Vascular drug effects
Nitric Oxide metabolism
Renal Artery cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0085-2538
- Volume :
- 65
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Kidney international
- Publication Type :
- Academic Journal
- Accession number :
- 14717929
- Full Text :
- https://doi.org/10.1111/j.1523-1755.2004.00408.x