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Specific oligonucleotide primers for identification of Cladophialophora carrionii, a causative agent of chromoblastomycosis.

Authors :
Abliz P
Fukushima K
Takizawa K
Nishimura K
Source :
Journal of clinical microbiology [J Clin Microbiol] 2004 Jan; Vol. 42 (1), pp. 404-7.
Publication Year :
2004

Abstract

Cladophialophora carrionii is one of the relatively common causative agents of chromoblastomycosis. We have developed the specific oligonucleotide primer set based on the internal transcribed spacer regions of ribosomal DNA for the rapid identification of this pathogen. PCR with this primer set amplified a 362-bp amplicon from C. carrionii strains. From other relevant dematiaceous species, including medically important dematiaceous fungi, such as Fonsecaea pedrosoi, Phialophora verrucosa, and Exophiala dermatitidis, and eight species of medically important yeasts, such as Candida albicans and Cryptococcus neoformans var. neoformans, the primer set did not produce any amplicon. PCR with this primer set may be a useful tool for the identification of C. carrionii.

Details

Language :
English
ISSN :
0095-1137
Volume :
42
Issue :
1
Database :
MEDLINE
Journal :
Journal of clinical microbiology
Publication Type :
Academic Journal
Accession number :
14715791
Full Text :
https://doi.org/10.1128/JCM.42.1.404-407.2004