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Species identification of all eight human herpesviruses with a single nested PCR assay.
- Source :
-
Journal of virological methods [J Virol Methods] 2004 Mar 01; Vol. 116 (1), pp. 19-26. - Publication Year :
- 2004
-
Abstract
- There are eight currently known human herpesviruses, all of which are capable of latent persistence and reactivation following primary infection. Herpesvirus induced disease is common, widespread, and associated with significant morbidity, particularly in the immunocompromised human host. Current methods of herpesvirus detection include viral culture and polymerase chain reaction (PCR). A robust PCR method based upon amplification of the highly conserved herpesvirus DNA polymerase gene that is capable of detection of all eight human herpesviruses, including EBV and HHV-6 subtypes, from clinical material is described. Species identification of PCR products is accomplished by either of two methods, chemiluminescent dot blot hybridization and heteroduplex mobility shift assay, both of which allow for simultaneous detection of multiple herpesviruses. This method should prove useful for rapid and accurate species identification of all eight human herpesviruses from clinical material.
- Subjects :
- Cytomegalovirus genetics
Cytomegalovirus isolation & purification
DNA Primers
DNA-Directed DNA Polymerase genetics
Electrophoretic Mobility Shift Assay
Herpesviridae genetics
Herpesvirus 1, Human genetics
Herpesvirus 1, Human isolation & purification
Herpesvirus 2, Human genetics
Herpesvirus 2, Human isolation & purification
Herpesvirus 3, Human genetics
Herpesvirus 3, Human isolation & purification
Herpesvirus 4, Human genetics
Herpesvirus 4, Human isolation & purification
Herpesvirus 6, Human genetics
Herpesvirus 6, Human isolation & purification
Herpesvirus 7, Human genetics
Herpesvirus 7, Human isolation & purification
Herpesvirus 8, Human genetics
Herpesvirus 8, Human isolation & purification
Heteroduplex Analysis methods
Humans
Nucleic Acid Hybridization methods
Herpesviridae classification
Herpesviridae isolation & purification
Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 0166-0934
- Volume :
- 116
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of virological methods
- Publication Type :
- Academic Journal
- Accession number :
- 14715303
- Full Text :
- https://doi.org/10.1016/j.jviromet.2003.10.005