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Immunocompetent hosts as a reservoir of pneumocystis organisms: histological and rt-PCR data demonstrate active replication.
- Source :
-
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2004 Feb; Vol. 23 (2), pp. 89-97. Date of Electronic Publication: 2004 Jan 08. - Publication Year :
- 2004
-
Abstract
- The present study was conducted to further examine recent data suggesting that pneumocystosis could be transmitted between patients and healthcare workers in the hospital environment, as has been proven with Pneumocystis-infected SCID mice and immunocompetent Balb/c mice. Using an experimental design (i.e., SCID-Balb/c mouse airborne transmission system), the present work found that healthy host-to-healthy host transmission of Pneumocystis organisms can occur, and that 'second' healthy contacts are able to transmit the infectious organisms to immunocompromised hosts. Further tests designed to explore the behavior of Pneumocystis organisms in the lungs of immunocompetent hosts were performed using histological and molecular approaches (e.g. testing the expression of both cyclin-dependent serine-threonine kinase and heat-shock 70 protein in Pneumocystis). The results showed Pneumocystis organisms were able to replicate in the lungs of immunocompetent hosts, which indicates these hosts are a reservoir for Pneumocystis spp.
- Subjects :
- Animals
Antibodies, Fungal analysis
Biopsy, Needle
DNA, Fungal analysis
Disease Models, Animal
Female
Immunocompromised Host immunology
Immunohistochemistry
Male
Mice
Mice, Inbred BALB C
Mice, SCID
Opportunistic Infections microbiology
Pneumonia, Pneumocystis immunology
Pneumonia, Pneumocystis pathology
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Species Specificity
Carrier State
Immunocompetence
Pneumocystis carinii isolation & purification
Pneumonia, Pneumocystis transmission
Subjects
Details
- Language :
- English
- ISSN :
- 0934-9723
- Volume :
- 23
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 14712369
- Full Text :
- https://doi.org/10.1007/s10096-003-1092-2