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Analysis of dissimilatory sulfite reductase and 16S rRNA gene fragments from deep-sea hydrothermal sites of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 2004 Jan; Vol. 70 (1), pp. 393-403. - Publication Year :
- 2004
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Abstract
- This study describes the occurrence of unique dissimilatory sulfite reductase (DSR) genes at a depth of 1,380 m from the deep-sea hydrothermal vent field at the Suiyo Seamount, Izu-Bonin Arc, Western Pacific, Japan. The DSR genes were obtained from microbes that grew in a catheter-type in situ growth chamber deployed for 3 days on a vent and from the effluent water of drilled holes at 5 degrees C and natural vent fluids at 7 degrees C. DSR clones SUIYOdsr-A and SUIYOdsr-B were not closely related to cultivated species or environmental clones. Moreover, samples of microbial communities were examined by PCR-denaturing gradient gel electrophoresis (DGGE) analysis of the 16S rRNA gene. The sequence analysis of 16S rRNA gene fragments obtained from the vent catheter after a 3-day incubation revealed the occurrence of bacterial DGGE bands affiliated with the Aquificae and gamma- and epsilon-Proteobacteria as well as the occurrence of archaeal phylotypes affiliated with the Thermococcales and of a unique archaeon sequence that clustered with "Nanoarchaeota." The DGGE bands obtained from drilled holes and natural vent fluids from 7 to 300 degrees C were affiliated with the delta-Proteobacteria, genus Thiomicrospira, and Pelodictyon. The dominant DGGE bands retrieved from the effluent water of casing pipes at 3 and 4 degrees C were closely related to phylotypes obtained from the Arctic Ocean. Our results suggest the presence of microorganisms corresponding to a unique DSR lineage not detected previously from other geothermal environments.
- Subjects :
- Amino Acid Sequence
Archaea classification
Archaea enzymology
Archaea isolation & purification
Base Sequence
DNA, Archaeal analysis
DNA, Bacterial analysis
DNA, Ribosomal analysis
Electrophoresis methods
Genes, rRNA
Molecular Sequence Data
Oxidoreductases Acting on Sulfur Group Donors chemistry
Pacific Ocean
Phylogeny
Polymerase Chain Reaction
Proteobacteria classification
Proteobacteria enzymology
Proteobacteria isolation & purification
Sequence Analysis, DNA
Thermococcales classification
Thermococcales enzymology
Thermococcales genetics
Archaea genetics
Hot Temperature
Oxidoreductases Acting on Sulfur Group Donors genetics
Proteobacteria genetics
RNA, Ribosomal, 16S genetics
Seawater microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0099-2240
- Volume :
- 70
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 14711668
- Full Text :
- https://doi.org/10.1128/AEM.70.1.393-403.2004