Back to Search
Start Over
hnRNP L enhances sensitivity of the cells to KW-2189.
hnRNP L enhances sensitivity of the cells to KW-2189.
- Source :
-
International journal of cancer [Int J Cancer] 2004 Feb 20; Vol. 108 (5), pp. 679-85. - Publication Year :
- 2004
-
Abstract
- Heterogeneous nuclear ribonucleoproteins (hnRNPs) are involved in several RNA-related biological processes. We demonstrated hnRNP L as a candidate protein of DARP (duocarmycin-DNA adduct recognizing protein) by gel shift assay and amino acid sequencing. Stable transfectants of hnRNP L showed high sensitivity of the cells to the growth inhibitory effect of KW-2189, a duocarmycin derivative in vitro. Immunostaining of hnRNP L demonstrated differential intracellular localization of hnRNP L among human lung cancer cell lines. A transfection study using a series of deletion mutants of hnRNP L fused to indicated that the N-terminal portions of RRM(RNA recognition motif)1, RRM3 and RRM2 are involved in localization of hnRNP L. We identified sequences in these portions that have high homology with the sequences of known NLS (nuclear localization signal) and NES (nuclear export signal). hnRNP L is a factor that determines the sensitivities of cancer cells to the minor groove binder, and overexpression and differential intracellular localization of hnRNP L are involved in its function in lung cancer.<br /> (Copyright 2003 Wiley-Liss, Inc.)
- Subjects :
- Amino Acid Sequence
Animals
DNA-Binding Proteins metabolism
Duocarmycins
Heterogeneous-Nuclear Ribonucleoprotein L pharmacology
Humans
Mice
Mutation
Nuclear Proteins metabolism
Ribonucleoproteins
Transfection
Tumor Cells, Cultured
Heterogeneous-Nuclear Ribonucleoprotein L metabolism
Lung Neoplasms metabolism
Pyrrolidinones pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0020-7136
- Volume :
- 108
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- International journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 14696094
- Full Text :
- https://doi.org/10.1002/ijc.11616