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Effects of Helicobacter pylori infection on the link between regenerating gene expression and serum gastrin levels in Mongolian gerbils.
- Source :
-
Laboratory investigation; a journal of technical methods and pathology [Lab Invest] 2003 Dec; Vol. 83 (12), pp. 1777-86. - Publication Year :
- 2003
-
Abstract
- Although regenerating gene (Reg) protein is reported to have a trophic effect on gastric epithelial cells, its involvement in human gastric diseases is not clear. We have recently shown that both gastrin and gastric mucosal inflammation enhance Reg gene expression in the fundic mucosa in rats. This study was designed to clarify whether Reg protein is involved in Helicobacter pylori-induced gastritis and whether Reg gene expression is linked to serum gastrin levels in this condition. Mongolian gerbils were inoculated with an H. pylori strain isolated from a gastric cancer patient. Four weeks later, some of the gerbils with H. pylori infection were eradicated by lansoprazole, amoxicillin, and clarithromycin. The time courses of changes in Reg gene expression, serum gastrin levels, gastric acidity, and histopathologic factors were examined. Four weeks after H. pylori infection, gastritis started spreading to the fundic mucosa, and gastric acidity started reducing. Serum gastrin levels and Reg mRNA expression in the fundus were significantly increased 6 weeks after infection. Reg mRNA expression in the fundus correlated significantly with both serum gastrin levels and the severity of fundic mucosal inflammation. After H. pylori eradication, serum gastrin levels and fundic mucosal inflammation were normalized, and the increase in Reg mRNA expression was abolished. The Reg gene is associated with hypergastrinemia and fundic mucosal inflammation and may be involved in H. pylori-induced gastritis.
- Subjects :
- Animals
Anti-Bacterial Agents therapeutic use
Apoptosis
Base Sequence
Calcium-Binding Proteins genetics
Cell Division
Disease Models, Animal
Gastric Acidity Determination
Gastric Mucosa pathology
Gastritis drug therapy
Gastritis metabolism
Gastritis pathology
Gerbillinae
Helicobacter Infections drug therapy
Helicobacter Infections pathology
Helicobacter pylori drug effects
Helicobacter pylori isolation & purification
Lithostathine
Male
Molecular Sequence Data
RNA, Messenger metabolism
Sequence Alignment
Up-Regulation
Calcium-Binding Proteins metabolism
Gastrins blood
Helicobacter Infections metabolism
Helicobacter pylori pathogenicity
Nerve Tissue Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0023-6837
- Volume :
- 83
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Laboratory investigation; a journal of technical methods and pathology
- Publication Type :
- Academic Journal
- Accession number :
- 14691296
- Full Text :
- https://doi.org/10.1097/01.lab.0000106501.56339.ce