Back to Search
Start Over
Amelioration of hippocampal neuronal damage after transient forebrain ischemia in cyclooxygenase-2-deficient mice.
- Source :
-
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism [J Cereb Blood Flow Metab] 2004 Jan; Vol. 24 (1), pp. 107-13. - Publication Year :
- 2004
-
Abstract
- Several studies have suggested that cyclooxygenase-2 (COX-2) plays a role in ischemic neuronal death. Genetic disruption of COX-2 has been shown to reduce susceptibility to focal ischemic injury and N-methyl-d-aspartate-mediated neurotoxicity. The purpose of this study was to examine the effects of COX-2 deficiency on neuronal vulnerability after transient forebrain ischemia. Marked upregulation of COX-2 immunostaining in neurons was observed at the early stage and prominent COX-2 staining persisted in the CA1 medial sector and CA2 sector over 3 days after ischemia. The immunohistologic pattern of COX-2 staining in these sectors gradually condensed to a perinuclear location. The degree of hippocampal neuronal injury produced by global ischemia in COX-2-deficient mice was less than that in wild-type mice, coincident with attenuation of DNA fragmentation in the hippocampus. Also, treatment with a selective COX-2 inhibitor, nimesulide, after ischemia decreased hippocampal neuronal damages. These results of genetic disruption and chemical inhibition of cyclooxygenase-2 show that inhibition of COX-2 ameliorates selective neuronal death after transient forebrain ischemia in mice.
- Subjects :
- Animals
Cell Death physiology
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
Cyclooxygenase Inhibitors pharmacology
DNA Fragmentation
Immunohistochemistry
Isoenzymes deficiency
Isoenzymes metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Prostaglandin-Endoperoxide Synthases deficiency
Prostaglandin-Endoperoxide Synthases metabolism
Sulfonamides pharmacology
Up-Regulation physiology
Hippocampus pathology
Ischemic Attack, Transient genetics
Ischemic Attack, Transient pathology
Isoenzymes genetics
Neurons pathology
Prostaglandin-Endoperoxide Synthases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0271-678X
- Volume :
- 24
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 14688622
- Full Text :
- https://doi.org/10.1097/01.WCB.0000100065.36077.4A