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Augmentation by 2-mercaptoethanol of in vitro anti-TNP antibody production induced by butyrate plus IL-2 in murine splenic B cells.
- Source :
-
Immunopharmacology and immunotoxicology [Immunopharmacol Immunotoxicol] 2003 Nov; Vol. 25 (4), pp. 539-50. - Publication Year :
- 2003
-
Abstract
- We previously reported that anti-trinitrophenyl (TNP) antibody production in murine splenic B cells stimulated with TNP-lipopolysaccharide in vitro was promoted by sodium butyrate (NaBu) in an IL-2-dependent manner. In the present study, we found that the effect of NaBu plus IL-2 was markedly augmented by 2-mercaptoethanol (2-ME), which showed a slight or null effect on the response of untreated, IL-2-treated or NaBu-treated B cells, as assessed by both anti-TNP plaque-forming cell assay and anti-TNP IgM ELISA. Other thiol compounds such as dithiothreitol, cysteamine and reduced glutathione (GSH) also had this activity. 2-ME enhanced the anti-TNP antibody production induced by other short-chain fatty acids with three to five carbon atoms plus IL-2. The proliferation of B cells was significantly inhibited by NaBu or NaBu plus IL-2, and the proliferation was completely restored by the simultaneous addition of 2-ME. These results demonstrate that 2-ME markedly enhanced anti-TNP antibody production in murine B cells induced by NaBu plus IL-2 and suggest that the effect of 2-ME is at least partly due to its blocking activity of the growth-inhibitory action of NaBu.
- Subjects :
- Acetylation
Animals
B-Lymphocytes cytology
Butyrates administration & dosage
Cell Division drug effects
Drug Synergism
Fatty Acids, Volatile administration & dosage
Fatty Acids, Volatile pharmacology
Female
Histones metabolism
In Vitro Techniques
Interleukin-2 administration & dosage
Mercaptoethanol administration & dosage
Mice
Mice, Inbred BALB C
Spleen cytology
Spleen immunology
Sulfhydryl Compounds administration & dosage
Sulfhydryl Compounds pharmacology
Antibody Formation drug effects
B-Lymphocytes drug effects
B-Lymphocytes immunology
Butyrates pharmacology
Interleukin-2 pharmacology
Lipopolysaccharides immunology
Mercaptoethanol pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0892-3973
- Volume :
- 25
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Immunopharmacology and immunotoxicology
- Publication Type :
- Academic Journal
- Accession number :
- 14686796
- Full Text :
- https://doi.org/10.1081/iph-120026439