Acid-induced denaturation of cellular retinol-binding proteins types I and II studied by electrospray mass spectrometry.

The acid-induced denaturation of cellular retinol-binding proteins types I and II (CRBP I and II), in the presence and in the absence of the ligand, was studied by electrospray ionization mass spectrometry (ESI-MS) in the pH range 6.9-2.4. To avoid artifacts generated by the ESI process, suitable interface parameters were selected. Different charge-state distributions were observed in the ESI-MS spectra, reflecting the pH-dependent equilibria among protein conformations in solution. In the absence of retinol, CRBP II appeared to be more resistant than CRBP I to acid denaturation. The bound ligand stabilized both carriers, with a markedly higher effect on CRBP I. Retinol release from the ligand-bound carriers and protein denaturation occurred concomitantly. This finding suggests that the lowering of pH, reported to occur in proximity to a biomembrane, might contribute to the conformational transitions required to promote dissociation of the otherwise very stable retinal-carrier complexes and thus permit targeted delivery of vitamin A to the enzymes involved in its metabolism.
(Copyright 2003 John Wiley & Sons, Ltd.)