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New reverse transcription-PCR assay for rapid and sensitive detection of enterovirus genomes in cerebrospinal fluid specimens of patients with aseptic meningitis.

New reverse transcription-PCR assay for rapid and sensitive detection of enterovirus genomes in cerebrospinal fluid specimens of patients with aseptic meningitis.

Authors :
Jacques J
Carquin J
Brodard V
Moret H
Lebrun D
Bouscambert M
Motte J
Rémy G
Andréoletti L
Source :
Journal of clinical microbiology [J Clin Microbiol] 2003 Dec; Vol. 41 (12), pp. 5726-8.
Publication Year :
2003

Abstract

Enterovirus (EV) detection by a new commercially available reverse transcription (RT)-PCR assay (Penter RT-PCR test) was compared with EV isolation from cell cultures and with EV detection by an in-house RT-PCR assay. Of the 54 cerebrospinal fluid specimens collected during a summer outbreak of aseptic meningitis, 52% were positive by cell culture versus 76% by in-house RT-PCR assay and 80% by the new RT-PCR test (52 versus 76 versus 80%; P = 0.003). This new reliable EV RNA detection test is suitable for clinical diagnosis of EV-related meningitis and may improve the management of EV-related neurological syndromes.

Details

Language :
English
ISSN :
0095-1137
Volume :
41
Issue :
12
Database :
MEDLINE
Journal :
Journal of clinical microbiology
Publication Type :
Academic Journal
Accession number :
14662967
Full Text :
https://doi.org/10.1128/JCM.41.12.5726-5728.2003