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New reverse transcription-PCR assay for rapid and sensitive detection of enterovirus genomes in cerebrospinal fluid specimens of patients with aseptic meningitis.
New reverse transcription-PCR assay for rapid and sensitive detection of enterovirus genomes in cerebrospinal fluid specimens of patients with aseptic meningitis.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2003 Dec; Vol. 41 (12), pp. 5726-8. - Publication Year :
- 2003
-
Abstract
- Enterovirus (EV) detection by a new commercially available reverse transcription (RT)-PCR assay (Penter RT-PCR test) was compared with EV isolation from cell cultures and with EV detection by an in-house RT-PCR assay. Of the 54 cerebrospinal fluid specimens collected during a summer outbreak of aseptic meningitis, 52% were positive by cell culture versus 76% by in-house RT-PCR assay and 80% by the new RT-PCR test (52 versus 76 versus 80%; P = 0.003). This new reliable EV RNA detection test is suitable for clinical diagnosis of EV-related meningitis and may improve the management of EV-related neurological syndromes.
- Subjects :
- Cerebrospinal Fluid virology
Enterovirus Infections cerebrospinal fluid
Humans
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Enterovirus genetics
Enterovirus isolation & purification
Enterovirus Infections diagnosis
Meningitis, Aseptic cerebrospinal fluid
Meningitis, Aseptic virology
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 41
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 14662967
- Full Text :
- https://doi.org/10.1128/JCM.41.12.5726-5728.2003