[Primary research of the effect of intestinal ischemia/reperfusion injury on Leptin concentrations].

Objective: To explore the effect of intestinal ischemia/reperfusion injury on Leptin concentrations in serum and adipose tissue, and to find out the role of Leptin in acute inflammatory responses.
Methods: An intestinal ischemia-reperfusion injury model of rats was established, and used a highly-sensitive murine Leptin radioimmunoassay to check the change of Leptin concentrations in serum and adipose tissue.
Results: Serum Leptin level (10.82+/-0.83) microg/L significantly decreased after an injury of 60-minute ischemia and 30-minute reperfusion versus pre-experimental serum levels (16.46+/-3.21) microg/L; Leptin level in serum was higher than that in adipose tissue (4.466+/-2.63) mg/100g, and they both showed a similar changeable trend to increase step by step as reperfusion time extended (P=0.047).
Conclusion: Leptin may be an inflammatory cytokine and may play a role in inflammatory responses such as intestinal ischemia/reperfusion.