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CatSper1 required for evoked Ca2+ entry and control of flagellar function in sperm.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2003 Dec 09; Vol. 100 (25), pp. 14864-8. Date of Electronic Publication: 2003 Dec 01. - Publication Year :
- 2003
-
Abstract
- CatSper family proteins are putative ion channels expressed exclusively in membranes of the sperm flagellum and required for male fertility. Here, we show that mouse CatSper1 is essential for depolarization-evoked Ca2+ entry and for hyperactivated movement, a key flagellar function. CatSper1 is not needed for other developmental landmarks, including regional distributions of CaV1.2, CaV2.2, and CaV2.3 ion channel proteins, the cAMP-mediated activation of motility by HCO3-, and the protein phosphorylation cascade of sperm capacitation. We propose that CatSper1 functions as a voltage-gated Ca2+ channel that controls Ca2+ entry to mediate the hyperactivated motility needed late in the preparation of sperm for fertilization.
- Subjects :
- Animals
Bicarbonates chemistry
Calcium Channels chemistry
Calcium Channels, L-Type chemistry
Calcium Channels, R-Type
Cyclic AMP metabolism
Immunoblotting
Immunohistochemistry
Ions
Male
Mice
Mice, Inbred C57BL
Microscopy, Confocal
Mutation
Phosphorylation
Time Factors
Tyrosine metabolism
Calcium metabolism
Calcium Channels physiology
Cation Transport Proteins
Flagella metabolism
Spermatozoa metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 100
- Issue :
- 25
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 14657352
- Full Text :
- https://doi.org/10.1073/pnas.2536658100