Blockade of paeoniflorin on sodium current in mouse hippocampal CA1 neurons.

Authors :: Zhang GQ
Hao XM
Chen SZ
Zhou PA
Cheng HP
Wu CH
Source :: Acta pharmacologica Sinica [Acta Pharmacol Sin] 2003 Dec; Vol. 24 (12), pp. 1248-52.
Publication Year :: 2003
Abstract: Aim: To study the blockade of paeoniflorin (Pae) on I(Na) in the acutely isolated hippocampus neurons of mice.<br />Methods: The whole-cell patch clamp technique was used.<br />Results: Pae inhibited I(Na) in frequency-dependent and concentration-dependent manners, with an IC50 of 271 micromol/L. Pae 0.3 mmol/L shifted the activation potential of the maximal I(Na) from -40 mV to -30 mV, shifted the steady-state activation and inactivation curves toward more positive and negative potentials by 10.8 mV, and 18.2 mV, respectively, and postponed the recovery of I(Na) inactivation state from (4.2+/-0.7) ms to (9.8+/-1.2) ms.<br />Conclusion: Pae inhibited I(Na) in mouse hippocampus neurons.