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Developmental regulation of phosphoprotein gene expression in the caudate-putamen of rat: an in situ hybridization study.
- Source :
-
Neuroscience [Neuroscience] 1992 Nov; Vol. 51 (1), pp. 65-75. - Publication Year :
- 1992
-
Abstract
- The regional and cellular ontogeny of the mRNA encoding the dopamine- and cAMP-regulated phosphoprotein, DARPP-32, has been studied in rat striatum by quantitative in situ hybridization histochemistry. The mRNA for DARPP-32 exhibited a characteristic developmental profile. The hybridization signal was first visible on the day of birth, at which time DARPP-32 mRNA was concentrated in patches in the caudate-putamen. By the end of the first postnatal week, the majority of neurons in the caudate-putamen expressed the DARPP-32 message. Levels of mRNA per cell increased markedly during the second postnatal week, and peaked around the beginning of the third week. The adult level of DARPP-32 mRNA was lower than that observed at the apex of mRNA expression, on a per cell basis, while the proportion of neurons expressing detectable levels of message remained relatively constant. In the nucleus accumbens and olfactory tubercle, DARPP-32 mRNA development lagged somewhat behind that observed in the caudate-putamen, but was similar in other respects. A non-quantitative study employing an oligonucleotide probe complementary to the mRNA encoding another cAMP-regulated phosphoprotein, ARPP-21, revealed a similar developmental sequence to DARPP-32. The present results suggest that for DARPP-32 mRNA, genetic and, possibly, environmental factors play a role in determining the developmental patterns observed.
- Subjects :
- Animals
Autoradiography
Base Sequence
Brain metabolism
Caudate Nucleus embryology
Caudate Nucleus growth & development
DNA genetics
Dopamine and cAMP-Regulated Phosphoprotein 32
Embryonic and Fetal Development
Gestational Age
In Situ Hybridization
Molecular Sequence Data
Nerve Tissue Proteins biosynthesis
Oligodeoxyribonucleotides
Organ Specificity
Phosphoproteins biosynthesis
Putamen embryology
Putamen growth & development
RNA, Messenger analysis
Rats
Rats, Sprague-Dawley
Sulfur Radioisotopes
Aging physiology
Caudate Nucleus metabolism
Gene Expression Regulation
Nerve Tissue Proteins genetics
Phosphoproteins genetics
Putamen metabolism
RNA, Messenger metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0306-4522
- Volume :
- 51
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 1465187
- Full Text :
- https://doi.org/10.1016/0306-4522(92)90471-d