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Co-amplification of ENFSI-loci D3S1358, D8S1179 and D18S51: validation of new primer sequences and allelic distribution among 2874 individuals.
- Source :
-
Forensic science international [Forensic Sci Int] 2003 Dec 17; Vol. 138 (1-3), pp. 104-10. - Publication Year :
- 2003
-
Abstract
- The present communication presents a new triplex PCR co-amplifying three loci (D3S1358, D8S1179 and D18S51) recommended for STR typing by the European Network of Forensic Science Institutes (ENFSI). Twenty-two different primers were tested to optimise the PCR. Four of the six primer sequences finally chosen were self selected, the fifth was a published one and the sixth derived from a commercially available multiplex kit. Using this PCR-setup, even minimum amounts of genomic DNA are sufficient to analyse the STR loci D3S1358, D8S1179 and D18S51 in parallel. Especially in forensic casework, where DNA is mostly limited and often contaminated with enzyme inhibitors, this new PCR proved to be very advantageous. To demonstrate the reliability, buccal swabs from 2874 persons were typed not only with the new triplex PCR but also with a commercially available multiplex kit.
- Subjects :
- Animals
Cats
Cattle
Chickens
Columbidae
Cricetinae
DNA isolation & purification
Dogs
Electrophoresis
Genetics, Population
Humans
Male
Mice
Pan troglodytes
Rabbits
Rats
Sheep
Species Specificity
Swine
DNA Fingerprinting methods
DNA Primers
Gene Frequency
Polymerase Chain Reaction methods
Sequence Analysis, DNA methods
Tandem Repeat Sequences
Subjects
Details
- Language :
- English
- ISSN :
- 0379-0738
- Volume :
- 138
- Issue :
- 1-3
- Database :
- MEDLINE
- Journal :
- Forensic science international
- Publication Type :
- Academic Journal
- Accession number :
- 14642726
- Full Text :
- https://doi.org/10.1016/j.forsciint.2003.09.007