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nm23-H1 reduces in vitro cell migration and the liver metastatic potential of colon cancer cells by regulating myosin light chain phosphorylation.
- Source :
-
International journal of cancer [Int J Cancer] 2004 Jan 10; Vol. 108 (2), pp. 207-11. - Publication Year :
- 2004
-
Abstract
- The nm23-H1 gene is known as a potential metastasis suppressor gene in various types of carcinomas. However, the role of nm23-H1 in colorectal carcinoma still remains controversial and the cellular mechanisms by which its protein may modulate the metastatic phenotype are not yet known. We transfected nm23-H1 cDNA into the human colon cancer cell line, HT-29, to test the effects and cellular biological mechanism of nm23 protein in colon cancer. We found that nm23-H1 strongly inhibited the liver metastasis of HT-29 cells in nude mice and inhibited the epidermal growth factor (EGF)-induced cell migration in vitro. Furthermore, we clarified the regulation of the myosin light chain (MLC) phosphorylation by nm23-H1, which has been demonstrated as having potential role in cell migration.<br /> (Copyright 2003 Wiley-Liss, Inc.)
- Subjects :
- Animals
Biomarkers, Tumor metabolism
Cell Movement drug effects
Colonic Neoplasms metabolism
Colonic Neoplasms pathology
Enzyme Activation
Epidermal Growth Factor pharmacology
Humans
In Vitro Techniques
Liver Neoplasms metabolism
Mice
Mice, Nude
Mitogen-Activated Protein Kinase Kinases metabolism
Monomeric GTP-Binding Proteins genetics
NM23 Nucleoside Diphosphate Kinases
Phosphorylation
Transcription Factors genetics
Transfection
Tumor Cells, Cultured
Cell Movement physiology
Colonic Neoplasms prevention & control
Liver Neoplasms prevention & control
Liver Neoplasms secondary
Monomeric GTP-Binding Proteins physiology
Myosin Light Chains metabolism
Nucleoside-Diphosphate Kinase
Transcription Factors physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0020-7136
- Volume :
- 108
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 14639604
- Full Text :
- https://doi.org/10.1002/ijc.11546