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Genomic structure and transcriptional regulation of Che-1, a novel partner of Rb.
- Source :
-
Gene [Gene] 2003 Dec 04; Vol. 321, pp. 57-63. - Publication Year :
- 2003
-
Abstract
- We recently identified and cloned a novel human gene, Che-1, whose product interacts with both RNA polymerase II and the retinoblastoma gene product (Rb). Furthermore, we found that Che-1 overexpression counteracts the growth inhibitory effects of Rb, regulating in such way both transcription and cell proliferation. In this paper, we describe the genomic organization of the mouse orthologous Che-1 gene and its promoter region. The promoter is TATA less and presents several potential transcription factor-binding motifs. Importantly, we showed that Che-1 expression is regulated by a negative feedback mechanism, in which this protein is present on its own promoter repressing transcription.
- Subjects :
- Amino Acid Sequence
Animals
Apoptosis Regulatory Proteins
Base Sequence
Cells, Cultured
Cloning, Molecular
DNA chemistry
DNA genetics
DNA, Complementary chemistry
DNA, Complementary genetics
Exons
Female
Gene Expression Regulation
Humans
Introns
Luciferases genetics
Luciferases metabolism
Male
Mice
Molecular Sequence Data
NIH 3T3 Cells
Promoter Regions, Genetic genetics
Protein Binding
RNA, Messenger genetics
RNA, Messenger metabolism
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Repressor Proteins metabolism
Sequence Analysis, DNA
Transcription Factors metabolism
Transcription, Genetic
Transfection
Genes genetics
Repressor Proteins genetics
Retinoblastoma Protein metabolism
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1119
- Volume :
- 321
- Database :
- MEDLINE
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 14636992
- Full Text :
- https://doi.org/10.1016/s0378-1119(03)00834-5