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Changes in cellular composition of kidney collecting duct cells in rats with lithium-induced NDI.
- Source :
-
American journal of physiology. Cell physiology [Am J Physiol Cell Physiol] 2004 Apr; Vol. 286 (4), pp. C952-64. Date of Electronic Publication: 2003 Nov 12. - Publication Year :
- 2004
-
Abstract
- Lithium treatment for 4 wk caused severe polyuria, dramatic downregulation in aquaporin-2 (AQP-2) expression, and marked decrease in AQP-2 immunoreactivity with the appearance of a large number of cells without AQP-2 labeling in the collecting ducts after lithium treatment. Surprisingly, this was not all due to an increase in AQP-2-negative principal cells, because double immunolabeling revealed that the majority of the AQP-2-negative cells displayed [H(+)]ATPase labeling, which identified them as intercalated cells. Moreover, multiple [H(+)]ATPase-labeled cells were adjacent, which was never seen in control rats. Quantitation confirmed a significant decrease in the fraction of collecting duct cells that exhibited detectable AQP-2 labeling compared with control rats: in cortical collecting ducts, 40 +/- 3.4 vs. 62 +/- 1.8% of controls (P < 0.05; n = 4) and in inner medullary collecting ducts, 58 +/- 1.6 vs. 81 +/- 1.3% of controls (P < 0.05; n = 4). In parallel, a significant increase in the fraction of intercalated ([H(+)]ATPase-positive) cells was shown. Urine output, whole kidney AQP-2 expression, cellular organization, and the fractions of principal and intercalated cells in cortex and inner medulla returned to control levels after 4 wk on a lithium-free diet following 4 wk on a lithium-containing diet. In conclusion, lithium treatment not only decreased AQP-2 expression, but dramatically and reversibly reduced the fraction of principal cells and altered the cellular organization in collecting ducts. These effects are likely to be important in lithium-induced nephrogenic diabetes insipidus.
- Subjects :
- Animals
Antibodies
Aquaporin 2
Aquaporin 4
Aquaporin 6
Aquaporins immunology
Aquaporins metabolism
Carrier Proteins immunology
Carrier Proteins metabolism
Down-Regulation drug effects
Kidney Tubules, Collecting ultrastructure
Microscopy, Immunoelectron
Proton-Translocating ATPases immunology
Proton-Translocating ATPases metabolism
Rats
Rats, Wistar
Recovery of Function
Sulfate Transporters
Urine
Diabetes Insipidus, Nephrogenic chemically induced
Diabetes Insipidus, Nephrogenic metabolism
Kidney Tubules, Collecting drug effects
Kidney Tubules, Collecting metabolism
Lithium pharmacology
Membrane Transport Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0363-6143
- Volume :
- 286
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Cell physiology
- Publication Type :
- Academic Journal
- Accession number :
- 14613889
- Full Text :
- https://doi.org/10.1152/ajpcell.00266.2003