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Phage integrases for the construction and manipulation of transgenic mammals.
- Source :
-
Reproductive biology and endocrinology : RB&E [Reprod Biol Endocrinol] 2003 Nov 07; Vol. 1, pp. 79. Date of Electronic Publication: 2003 Nov 07. - Publication Year :
- 2003
-
Abstract
- Phage integrases catalyze site-specific, unidirectional recombination between two short att recognition sites. Recombination results in integration when the att sites are present on two different DNA molecules and deletion or inversion when the att sites are on the same molecule. Here we demonstrate the ability of the phiC31 integrase to integrate DNA into endogenous sequences in the mouse genome following microinjection of donor plasmid and integrase mRNA into mouse single-cell embryos. Transgenic early embryos and a mid-gestation mouse are reported. We also demonstrate the ability of the phiC31, R4, and TP901-1 phage integrases to recombine two introduced att sites on the same chromosome in human cells, resulting in deletion of the intervening material. We compare the frequencies of mammalian chromosomal deletion catalyzed by these three integrases in different chromosomal locations. The results reviewed here introduce these bacteriophage integrases as tools for site-specific modification of the genome for the creation and manipulation of transgenic mammals.
- Subjects :
- Animals
Attachment Sites, Microbiological genetics
Base Sequence
Cell Line
Chromosome Deletion
Genome
Humans
Mammals embryology
Mice
Mice, Transgenic
Mutagenesis, Site-Directed genetics
Bacillus Phages enzymology
Gene Targeting methods
Integrases metabolism
Mammals metabolism
Recombination, Genetic genetics
Transgenes genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1477-7827
- Volume :
- 1
- Database :
- MEDLINE
- Journal :
- Reproductive biology and endocrinology : RB&E
- Publication Type :
- Academic Journal
- Accession number :
- 14613545
- Full Text :
- https://doi.org/10.1186/1477-7827-1-79