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Cyclin D2 controls B cell progenitor numbers.
- Source :
-
Journal of leukocyte biology [J Leukoc Biol] 2003 Dec; Vol. 74 (6), pp. 1139-43. Date of Electronic Publication: 2003 Nov 11. - Publication Year :
- 2003
-
Abstract
- Cyclin D2 affects B cell proliferation and differentiation in vivo. It is rate-limiting for B cell receptor (BCR)-dependent proliferation of B cells, and cyclin D2-/- mice lack CD5+(B1) B lymphocytes. We show here that the bone marrow (BM) of cyclin D2-/- mice contains half the numbers of Sca1+B220+ B cell progenitors but normal levels of Sca1+ progenitor cells of other lineages. In addition, clonal analysis of BM from the cyclin D2-/- and cyclin D2+/+ mice confirmed that there were fewer B cell progenitors (B220+) in the cyclin D2-/- mice. In addition, the colonies from cyclin D2-/- mice were less mature (CD19lo) than those from cyclin D2+/+ mice (CD19Hi). The number of mature B2 B cells in vivo is the same in cyclin D2-/- and cyclin D2+/+ animals. Lack of cyclin D2 protein may be compensated by cyclin D3, as cyclin-dependent kinase (cdk)6 coimmunoprecipitates with cyclin D3 but not cyclin D1 from BM mononuclear cells of cyclin D2-/- mice. It is active, as endogenous retinoblastoma protein is phosphorylated at the cdk6/4-cyclin D-specific sites, S807/811. We conclude that cyclin D2 is rate-limiting for the production of B lymphoid progenitor cells whose proliferation does not depend on BCR signaling.
- Subjects :
- Animals
Antigens, CD19 metabolism
Antigens, Differentiation metabolism
B-Lymphocytes drug effects
Blotting, Western
Bone Marrow metabolism
CD5 Antigens analysis
CD5 Antigens metabolism
Cell Count
Cells, Cultured
Colony-Forming Units Assay
Cyclin D2
Cyclin D3
Cyclins metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Oncogene Proteins metabolism
Phosphorylation
Proto-Oncogene Proteins c-bcr
Retinoblastoma Protein metabolism
Signal Transduction
B-Lymphocytes cytology
Cyclins physiology
Hematopoietic Stem Cells cytology
Protein-Tyrosine Kinases
Proto-Oncogene Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0741-5400
- Volume :
- 74
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of leukocyte biology
- Publication Type :
- Academic Journal
- Accession number :
- 14612431
- Full Text :
- https://doi.org/10.1189/jlb.0803363