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Identification of estrogen-responsive genes by complementary deoxyribonucleic acid microarray and characterization of a novel early estrogen-induced gene: EEIG1.
- Source :
-
Molecular endocrinology (Baltimore, Md.) [Mol Endocrinol] 2004 Feb; Vol. 18 (2), pp. 402-11. Date of Electronic Publication: 2003 Nov 06. - Publication Year :
- 2004
-
Abstract
- Estrogen receptors (ERs) are nuclear transcription factors that regulate gene expression in response to estrogen and estrogen-like compounds. Identification of estrogen-regulated genes in target cells is an essential step toward understanding the molecular mechanisms of estrogen action. Using cDNA microarray examinations, 19 genes were identified as induced by 17 beta-estradiol in MCF-7 cells, 10 of which have been reported previously to be estrogen responsive or to be linked with ER status. Five known estrogen-regulated genes, E2IG4, IGFBP4, SLC2A1, XBP1 and B4GALT1, and AFG3L1, responded quickly to estrogen treatment. A novel estrogen-responsive gene was identified and named EEIG1for early estrogen-induced gene 1. EEIG1 was clearly induced by 17 beta-estradiol within 2 h of treatment, and was widely responsive to a group of estrogenic compounds including natural and synthetic estrogens and estrogenic environmental compounds. EEIG1 was expressed in ER-positive but not in ER-negative breast cancer cell lines. EEIG1 expression was repressed by antiestrogens 4-OH-tamoxifen and ICI 182,780 but not by protein synthesis inhibitors cycloheximide and puromycin. These results provide evidence that some estrogenic compounds differentially enhance the transcription of estrogen-regulated genes and suggest a role for EEIG1 in estrogen action.
- Subjects :
- Breast Neoplasms genetics
Cycloheximide pharmacology
DNA-Binding Proteins genetics
Dose-Response Relationship, Drug
Estradiol pharmacology
Estrogen Antagonists pharmacology
Female
Fulvestrant
Galactosyltransferases drug effects
Galactosyltransferases genetics
Humans
Insulin-Like Growth Factor Binding Protein 4 drug effects
Insulin-Like Growth Factor Binding Protein 4 genetics
Neoplasm Proteins drug effects
Nuclear Proteins genetics
Pesticides pharmacology
Phytoestrogens pharmacology
Protein Synthesis Inhibitors pharmacology
Puromycin pharmacology
Receptors, Estrogen drug effects
Receptors, Estrogen genetics
Receptors, Estrogen metabolism
Regulatory Factor X Transcription Factors
Tamoxifen pharmacology
Transcription Factors
Tumor Cells, Cultured
X-Box Binding Protein 1
Estradiol analogs & derivatives
Estrogens pharmacology
Gene Expression Regulation drug effects
Neoplasm Proteins genetics
Oligonucleotide Array Sequence Analysis methods
Tamoxifen analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 0888-8809
- Volume :
- 18
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular endocrinology (Baltimore, Md.)
- Publication Type :
- Academic Journal
- Accession number :
- 14605097
- Full Text :
- https://doi.org/10.1210/me.2003-0202