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The molecular biology of Euglena gracilis. XV. Recovery from centrifugation-induced stratification.
- Source :
-
Cell motility and the cytoskeleton [Cell Motil Cytoskeleton] 2003 Dec; Vol. 56 (4), pp. 219-24. - Publication Year :
- 2003
-
Abstract
- The contents of Euglena gracilis cells can be separated in vivo by ultracentrifugation. Within the unbroken cell, each set of components forms a distinct layer according to their respective densities. The degree of segregation increases with both the g-force and the time of centrifugation, up to a maximum at 100,000 x g for 1 h, when six distinct strata can be observed. When returned to normal growth conditions, essentially all the cells return to the normal state and growth pattern. Greater g-forces or longer exposures do not alter the observable strata, but the ability of the cells to recover is diminished. Smaller g-forces result in less separation of cellular contents and all cells recover, even after 18 h of exposure. Euglena cells stratified at 100,000 x g for 1 h were returned to normal growth conditions; recovery was followed microscopically and by the rate of utilization of oxygen as well as that of the single carbon source. The cells recovered their normal state within 1 to 2 h, which is only a tenth of the normal doubling time. The mechanism for this recovery involves a natural process of change in cell shape caused by contraction and relaxation of the pellicle, a cell surface structure.
Details
- Language :
- English
- ISSN :
- 0886-1544
- Volume :
- 56
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cell motility and the cytoskeleton
- Publication Type :
- Academic Journal
- Accession number :
- 14584024
- Full Text :
- https://doi.org/10.1002/cm.10145