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Is the cytomegalovirus serologic status always accurate? A comparative analysis of humoral and cellular immunity.
- Source :
-
Transplantation [Transplantation] 2003 Oct 27; Vol. 76 (8), pp. 1229-30. - Publication Year :
- 2003
-
Abstract
- Background: The precise knowledge of cytomegalovirus (CMV) status is important in a variety of clinical settings such as transplantation or blood transfusion. There are, however, various situations where an immunoglobulin-based diagnostic approach has limitations.<br />Methods: The CMV status of 388 individuals was determined by the analysis of both CMV-specific immunoglobulins and cellular immunity using a standard enzyme-linked immunosorbent assay and a rapid-flow cytometric approach, respectively.<br />Results: In 386 of 388 individuals, both assays yielded concordant results in assigning the CMV status (294 positive and 92 negative). In 2 of 94 (2.1%) CMV immunoglobulin (Ig) G-negative individuals, CMV-specific CD4 T cells were detectable. Repeated testing of the index and follow-up samples yielded one positive and one marginally positive IgG titer.<br />Conclusions: Both assays yielded highly concordant results. Thus, the T-cell-based approach may represent a reliable alternative for defining CMV status in any clinical situation where serologic testing is limited by borderline titers or by the presence of passively administered antibodies.
Details
- Language :
- English
- ISSN :
- 0041-1337
- Volume :
- 76
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Transplantation
- Publication Type :
- Academic Journal
- Accession number :
- 14578758
- Full Text :
- https://doi.org/10.1097/01.TP.0000083894.61333.56