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[Expression of telomerase reverse transcriptase in premalignant esophageal squamous dysplasia].
- Source :
-
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi [Zhonghua Liu Xing Bing Xue Za Zhi] 2003 Oct; Vol. 24 (10), pp. 920-3. - Publication Year :
- 2003
-
Abstract
- Objective: To study the relationship of human telomerase reverse transcriptase (hTRT) and malignant transformation of esophageal dysplasia.<br />Methods: Telomerase activity and hTRT expression in esophageal dysplasia (n = 47), squamous cell carcinoma (n = 29) and normal esophagus (n = 11) were detected by telomeric repeat amplification protocol (TRAP) and in situ hybridization, respectively.<br />Results: Telomerase activity was detected in none of the 11 cases of normal esophageal tissues (0%) but in 21 of 47 cases (44.7%) of dysplasia, and in 25 of 29 cases (86.2%) of esophageal squamous cell carcinoma. There were statistically significant differences among the telomerase activity in normal esophagus, esophageal dysplasia, and in squamous cell carcinoma (chi(2) = 5.89, P < 0.05; chi(2) = 11.35, P < 0.01). hTRT mRNA was expressed in none of the 11 cases of normal esophageal tissues (0%) but in 23 of 47 cases (48.9%) of dysplasia, and in 24 of 29 cases (82.8%) of esophageal squamous cell carcinoma. There were statistically significant differences among the expression of hTRT mRNA in normal esophagus, esophageal dysplasia, and in squamous cell carcinoma (chi(2) = 6.99, P < 0.01; chi(2) = 7.32, P < 0.01). Significant correlation was found between the telomerase activity and the expression of hTRT mRNA (chi(2) = 57.91, P < 0.001).<br />Conclusion: The mRNA expression of hTRT which paralleled to telomerase activity implied that there was a crucial role to play in regulating the activation of telomerase, and was closely related to the malignant transformation of esophageal dysplasia. hTRT might serve as a new, valuable biomarker to detect esophageal squamous cell carcinoma.
- Subjects :
- Adult
Aged
Biomarkers, Tumor analysis
DNA-Binding Proteins
Esophageal Neoplasms pathology
Esophagus pathology
Female
Humans
Male
Middle Aged
Precancerous Conditions pathology
RNA, Messenger analysis
Telomerase metabolism
Esophageal Neoplasms enzymology
Precancerous Conditions enzymology
Telomerase genetics
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0254-6450
- Volume :
- 24
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi
- Publication Type :
- Academic Journal
- Accession number :
- 14575609