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Cytochrome P450 2A6: a new hepatic autoantigen in patients with chronic hepatitis C virus infection.
- Source :
-
Journal of hepatology [J Hepatol] 2003 Nov; Vol. 39 (5), pp. 800-6. - Publication Year :
- 2003
-
Abstract
- Background/aims: Cytochromes P4502A6 (CYP2A6) and P4501A2 (CYP1A2) were described as hepatic autoantigens in the autoimmune polyglandular syndrome type-1 (APS-1). We evaluated the significance of anti-CYP2A6 and anti-CYP1A2 in several hepatic diseases in the absence of APS-1.<br />Methods: A radioligand assay (RLA) based on immunoprecipitation of [(35)S]-methionine-labeled CYP2A6 and CYP1A2 was used. Four hundred and thirty subjects with chronic viral hepatitis (n=185), autoimmune liver diseases (n=181), autoimmune rheumatic diseases (ARD, n=31) and healthy (n=33) were tested.<br />Results: Seven out of 366 patients with liver diseases were anti-CYP2A6 positive. Neither healthy nor ARD patients showed anti-CYP2A6. One out of 181 patients with autoimmune liver diseases tested anti-CYP2A6 positive. A significantly higher prevalence of anti-CYP2A6 (P<0.05) was detected with six out of seven patients positive in the viral hepatitis group. The latter were infected by flaviviruses (1 HGV/GBVC, 5 HCV). 4/5 HCV/anti-CYP2A6 positive sera were positive for anti-LKM-1 by immunofluorescence and for anti-CYP2D6 by RLA. None of the 430 sera recognized CYP1A2.<br />Conclusions: For the first time CYP2A6 is reported as a hepatic autoantigen in patients with viral hepatitis caused by flaviviruses and in particular in HCV/anti-LKM-1 positive patients. Multicenter studies are needed in order to investigate the clinical importance of this novel finding. This study further supports that anti-CYP2A6 in the absence of flavivirus is rather limited to APS-1.
- Subjects :
- Adolescent
Adult
Autoantibodies analysis
Autoimmune Diseases immunology
Case-Control Studies
Child
Chronic Disease
Cytochrome P-450 CYP2A6
Female
Flaviviridae Infections immunology
Hepatitis, Viral, Human immunology
Humans
Liver Diseases immunology
Male
Middle Aged
Rheumatic Diseases immunology
Aryl Hydrocarbon Hydroxylases immunology
Autoantigens immunology
Hepatitis C, Chronic immunology
Mixed Function Oxygenases immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0168-8278
- Volume :
- 39
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of hepatology
- Publication Type :
- Academic Journal
- Accession number :
- 14568264
- Full Text :
- https://doi.org/10.1016/s0168-8278(03)00356-8