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Determination of monosaccharides and sugar alcohols in tissues from diabetic rats by high-performance liquid chromatography with pulsed amperometric detection.

Authors :
Tomiya N
Suzuki T
Awaya J
Mizuno K
Matsubara A
Nakano K
Kurono M
Source :
Analytical biochemistry [Anal Biochem] 1992 Oct; Vol. 206 (1), pp. 98-104.
Publication Year :
1992

Abstract

A sensitive and simple high-performance liquid chromatographic method has been developed to determine the concentration of monosaccharides and sugar alcohols in animal tissues. Five neutral monosaccharides (D-glucose, D-galactose, D-mannose, D-fructose, and D-ribose) and three neutral sugar alcohols (myo-inositol, glycerol, and D-sorbitol) predominate in the renal cortices and sciatic nerves of rats. These monosaccharides and sugar alcohols were extracted with distilled water, purified by deproteinization with ethanol, a Sep-Pak C18 cartridge, and columns of Dowex 50W-X8 and Amberlite CG-400, then separated on Ca2+ and Pb2+ cation-exchange columns, eluted with deionized distilled water at 80 degrees C, and detected using integrated pulsed amperometry. About 10 pmol of each sugar was detectable with a signal-to-noise ratio of 10:1. D-Glucose, D-fructose, D-sorbitol, and D-mannose were higher in both the renal and sciatic tissues of diabetic rats than in those of normal animals. D-Ribose and glycerol were higher in the renal cortex of diabetic animals.

Details

Language :
English
ISSN :
0003-2697
Volume :
206
Issue :
1
Database :
MEDLINE
Journal :
Analytical biochemistry
Publication Type :
Academic Journal
Accession number :
1456448
Full Text :
https://doi.org/10.1016/s0003-2697(05)80016-8