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Determination of monosaccharides and sugar alcohols in tissues from diabetic rats by high-performance liquid chromatography with pulsed amperometric detection.
- Source :
-
Analytical biochemistry [Anal Biochem] 1992 Oct; Vol. 206 (1), pp. 98-104. - Publication Year :
- 1992
-
Abstract
- A sensitive and simple high-performance liquid chromatographic method has been developed to determine the concentration of monosaccharides and sugar alcohols in animal tissues. Five neutral monosaccharides (D-glucose, D-galactose, D-mannose, D-fructose, and D-ribose) and three neutral sugar alcohols (myo-inositol, glycerol, and D-sorbitol) predominate in the renal cortices and sciatic nerves of rats. These monosaccharides and sugar alcohols were extracted with distilled water, purified by deproteinization with ethanol, a Sep-Pak C18 cartridge, and columns of Dowex 50W-X8 and Amberlite CG-400, then separated on Ca2+ and Pb2+ cation-exchange columns, eluted with deionized distilled water at 80 degrees C, and detected using integrated pulsed amperometry. About 10 pmol of each sugar was detectable with a signal-to-noise ratio of 10:1. D-Glucose, D-fructose, D-sorbitol, and D-mannose were higher in both the renal and sciatic tissues of diabetic rats than in those of normal animals. D-Ribose and glycerol were higher in the renal cortex of diabetic animals.
- Subjects :
- Animals
Carbohydrates isolation & purification
Chromatography, High Pressure Liquid methods
Electrochemistry methods
Kidney Cortex chemistry
Kidney Cortex metabolism
Male
Monosaccharides isolation & purification
Monosaccharides metabolism
Rats
Rats, Sprague-Dawley
Sciatic Nerve chemistry
Sciatic Nerve metabolism
Sugar Alcohols isolation & purification
Sugar Alcohols metabolism
Diabetes Mellitus, Experimental metabolism
Monosaccharides analysis
Sugar Alcohols analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0003-2697
- Volume :
- 206
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Analytical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 1456448
- Full Text :
- https://doi.org/10.1016/s0003-2697(05)80016-8