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Intraperitoneal transplantation of pancreatic anlagen.
- Source :
-
ASAIO journal (American Society for Artificial Internal Organs : 1992) [ASAIO J] 2003 Sep-Oct; Vol. 49 (5), pp. 527-32. - Publication Year :
- 2003
-
Abstract
- To determine whether embryonic pancreatic anlagen transplanted to an intraperitoneal site in adult hosts grow, differentiate, and function, we implanted pancreas from embryonic day (E) 12.5 Lewis rat embryos into the omentum of adult Lewis rats or C57Bl/6J mice. E12.5 pancreatic anlagen were relatively undifferentiated except for the presence of condensing tubuloacinar cords. By 2 weeks after implantation, pancreatic anlagen transplanted into rats had enlarged and differentiated such that islets of Langerhans that stained positive for insulin could be delineated. Continued differentiation, as reflected by the presence of "ductal" islets connected to the duct epithelium, was observed at 6 weeks after implantation. At 15 weeks after implantation, "mature" islets had separated from the ducts. Electron microscopy showed eccentric dense bodies within clear vacuoles consistent with insulin granules. Little or no acinar tissue was present in developed anlagen. Within 5 weeks of pancreatic anlagen transplantation, levels of glucose in rats rendered diabetic by an injection of streptozotocin were normalized compared with levels in nontransplanted diabetic controls. Rat pancreatic anlagen underwent growth and development in the peritoneum of C57Bl/61 mice that received costimulatory blocking agents but not in the absence of costimulatory blockade. We concluded that whole E12.5 pancreatic anlagen undergo growth, differentiation, and function after intraperitoneal placement. Implantation of the embryonic pancreas, a "cellular" transplant, is followed by selective differentiation of islet compared with acinar components.
- Subjects :
- Animals
Cell Differentiation
Cell Division
Islets of Langerhans cytology
Islets of Langerhans physiology
Mice
Mice, Inbred C57BL
Peritoneal Cavity
Rats
Rats, Inbred Lew
Transplantation, Heterologous
Fetal Tissue Transplantation
Graft Survival
Islets of Langerhans embryology
Omentum surgery
Pancreas Transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 1058-2916
- Volume :
- 49
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- ASAIO journal (American Society for Artificial Internal Organs : 1992)
- Publication Type :
- Academic Journal
- Accession number :
- 14524558
- Full Text :
- https://doi.org/10.1097/01.mat.0000084174.33319.7f