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Establishment of memory for IL-10 expression in developing T helper 2 cells requires repetitive IL-4 costimulation and does not impair proliferation.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2003 Oct 14; Vol. 100 (21), pp. 12307-12. Date of Electronic Publication: 2003 Sep 26. - Publication Year :
- 2003
-
Abstract
- T helper (Th) lymphocytes can develop memory for the expression of particular cytokines, like IL-4 or IL-10, in that reexpression of those cytokines is independent of the original costimulatory signal IL-4 and depends only on T cell receptor stimulation. Here, we show that in the course of Th2 cell differentiation in vitro, IL-4 memory is established during primary activation of naïve Th cells, whereas the establishment of IL-10 memory requires repetitive stimulation of the Th cell with IL-4 and T cell receptor. Likewise, established IL-10 memory, maintained in the absence of further IL-4 signals, was observed in individual IL-10-producing cells generated from in vivo antigen-experienced CD62L(low) Th cells and isolated by using the newly developed cytometric cytokine secretion assay for IL-10. In naïve Th cells undergoing primary activation, the induction of both IL-4 and IL-10 memory requires DNA synthesis, but reexpression of the cytokine genes can occur throughout cell cycle. In in vitro polarized Th2 cell populations, Th cells with IL-4 or IL-10 memory do not differ in proliferative behavior. Populations of Th cells isolated from polarized Th2 cultures according to expression of IL-4 or IL-10 also do not differ in proliferative behavior. Their proliferation mainly depends on IL-2. Thus, effector memory Th lymphocytes with memory for IL-4 or IL-10 expression are not intrinsically impaired in their proliferative potential and can play an essential role in reactive immunological memory and its regulation.
- Subjects :
- Animals
Antigens administration & dosage
Cell Cycle
Cell Differentiation
Cell Separation
DNA biosynthesis
In Vitro Techniques
Interleukin-4 administration & dosage
Lymphocyte Activation
Mice
Mice, Inbred BALB C
Mice, Transgenic
Ovalbumin administration & dosage
Ovalbumin immunology
Peptide Fragments administration & dosage
Peptide Fragments immunology
Th2 Cells cytology
Th2 Cells metabolism
Immunologic Memory
Interleukin-10 biosynthesis
Interleukin-4 biosynthesis
Th2 Cells immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 100
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 14514890
- Full Text :
- https://doi.org/10.1073/pnas.2035254100