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Persistence of adhesive properties in Vibrio cholerae after long-term exposure to sea water.
- Source :
-
Environmental microbiology [Environ Microbiol] 2003 Oct; Vol. 5 (10), pp. 850-8. - Publication Year :
- 2003
-
Abstract
- The effect of exposure to artificial sea water (ASW) on the ability of classical Vibrio cholerae O1 cells to interact with chitin-containing substrates and human intestinal cells was studied. Incubation of vibrios in ASW at 5 degrees C and 18 degrees C resulted in two kinds of cell responses: the viable but non-culturable (VBNC) state (i.e. <0.1 colony forming unit ml-1) at 5 degrees C, and starvation (i.e. maintenance of culturability of the population) at 18 degrees C. The latter remained rod shaped and, after 40 days' incubation, presented a 47-58% reduction in the number of cells attached to chitin, a 48-53% reduction in the number of bacteria adhering to copepods, and a 48-54% reduction in the number of bacteria adhering to human cultured intestinal cells, compared to control cells not suspended in ASW. Bacteria suspended in ASW at 5 degrees C became coccoid and, after 40 days, showed 34-42% fewer cells attached to chitin, 52-55% fewer adhering to copep-ods, and 45-48% fewer cells adhering to intestinal cell monolayers, compared to controls. Sarkosyl-insoluble membrane proteins that bind chitin particles were isolated and analysed by SDS-PAGE. After 40 days incubation in ASW at both 5 degrees C and 18 degrees C vibrios expressed chitin-binding ligands similar to bacteria harvested in the stationary growth phase. It is concluded that as vibrios do not lose adhesive properties after long-term exposure to ASW, it is important to include methods for VBNC bacteria when testing environmental and clinical samples for purposes of public health safety.
- Subjects :
- Animals
Bacterial Proteins metabolism
Caco-2 Cells
Chitin metabolism
Copepoda metabolism
Copepoda microbiology
Detergents metabolism
Humans
Intestinal Mucosa cytology
Intestinal Mucosa metabolism
Membrane Proteins metabolism
Sarcosine metabolism
Temperature
Cell Adhesion physiology
Sarcosine analogs & derivatives
Seawater
Vibrio cholerae physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1462-2912
- Volume :
- 5
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 14510838
- Full Text :
- https://doi.org/10.1046/j.1462-2920.2003.00498.x