Detection of non-enterotoxigenic and enterotoxigenic Bacteroides fragilis in stool samples from children in São Paulo, Brazil.

Authors :: Krzyzanowsky F
Avila-Campos MJ
Source :: Revista do Instituto de Medicina Tropical de Sao Paulo [Rev Inst Med Trop Sao Paulo] 2003 Jul-Aug; Vol. 45 (4), pp. 225-7. Date of Electronic Publication: 2003 Sep 17.
Publication Year :: 2003
Abstract: Non-enterotoxigenic bacteria of the Bacteroides fragilis group and enterotoxigenic B. fragilis were identified from children with and without aqueous acute diarrhea. In this study, 170 stool samples from 96 children with and 74 without diarrhea were analyzed. Enterotoxin production and the toxin gene detection were detected by cytotoxicity assay on HT-29/C1 cells and by PCR, respectively. B. fragilis species was prevalent in both groups and enterotoxigenic B. fragilis strains were isolated from two children with diarrhea. More studies are important to evaluate the role of each bacteria of the B. fragilis group, including enterotoxigenic strains play in the diarrheal processes in children.

Subjects :: Bacteroides fragilis classification
Brazil
Case-Control Studies
Child
Child, Preschool
Feces microbiology
Female
Humans
Infant
Infant, Newborn
Male
Polymerase Chain Reaction
Bacteroides Infections microbiology
Bacteroides fragilis isolation & purification
Diarrhea microbiology
Enterotoxins biosynthesis

Full Text Access

Tools