Back to Search
Start Over
A seven-transmembrane RGS protein that modulates plant cell proliferation.
- Source :
-
Science (New York, N.Y.) [Science] 2003 Sep 19; Vol. 301 (5640), pp. 1728-31. - Publication Year :
- 2003
-
Abstract
- G protein-coupled receptors (GPCRs) at the cell surface activate heterotrimeric G proteins by inducing the G protein alpha (Galpha) subunit to exchange guanosine diphosphate for guanosine triphosphate. Regulators of G protein signaling (RGS) proteins accelerate the deactivation of Galpha subunits to reduce GPCR signaling. Here we identified an RGS protein (AtRGS1) in Arabidopsis that has a predicted structure similar to a GPCR as well as an RGS box with GTPase accelerating activity. Expression of AtRGS1 complemented the pheromone supersensitivity phenotype of a yeast RGS mutant, sst2Delta. Loss of AtRGS1 increased the activity of the Arabidopsis Galpha subunit, resulting in increased cell elongation in hypocotyls in darkness and increased cell production in roots grown in light. These findings suggest that AtRGS1 is a critical modulator of plant cell proliferation.
- Subjects :
- Alleles
Amino Acid Sequence
Arabidopsis genetics
Arabidopsis Proteins chemistry
Arabidopsis Proteins genetics
Cell Differentiation
Cell Membrane metabolism
Heterotrimeric GTP-Binding Proteins metabolism
Meristem metabolism
Mitosis
Molecular Sequence Data
Mutation
Open Reading Frames
Phenotype
Plant Roots cytology
Plant Roots growth & development
Plant Roots metabolism
Protein Precursors metabolism
Protein Structure, Tertiary
RGS Proteins chemistry
RGS Proteins genetics
Recombinant Fusion Proteins chemistry
Recombinant Fusion Proteins metabolism
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins metabolism
Transgenes
Arabidopsis cytology
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Cell Division
GTP-Binding Protein alpha Subunits
RGS Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 301
- Issue :
- 5640
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 14500984
- Full Text :
- https://doi.org/10.1126/science.1087790