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Parachlamydia acanthamoebae enters and multiplies within human macrophages and induces their apoptosis [corrected].
- Source :
-
Infection and immunity [Infect Immun] 2003 Oct; Vol. 71 (10), pp. 5979-85. - Publication Year :
- 2003
-
Abstract
- Parachlamydia acanthamoebae is an obligately intracellular bacterium that naturally infects free-living amoebae. It is a potential human pathogen and may survive in human macrophages. We studied P. acanthamoebae entry into, and multiplication within, human monocyte-derived macrophages. After 8 h of incubation, 80% of macrophages were infected with a mean of 3.8 P. acanthamoebae organisms per cell. Electron microscopy demonstrated that parachlamydiae were in an intracellular vacuole. After infection with living organisms, the number of parachlamydiae per macrophage increased 4 times from day 0 to day 4, whereas heat-inactivated parachlamydiae were eliminated during the same period. Quantitative PCR confirmed that P. acanthamoebae replicates within macrophages. Transcriptional activity of P. acanthamoeba was detected by reverse transcription-PCR targeting the gene encoding ADP-ATP translocase (tlc). P. acanthamoebae exerted a cytopathic effect on macrophages. When macrophages were infected with living bacteria, their number decreased significantly from day 0 to day 4 due to apoptosis, as shown by annexin-V binding and electron microscopy. This study shows that P. acanthamoebae enters and multiplies within human macrophages before inducing their apoptosis.
- Subjects :
- Apoptosis
Bacterial Proteins genetics
Base Sequence
Chlamydiales genetics
Chlamydiales growth & development
Chlamydiales ultrastructure
DNA, Bacterial genetics
Genes, Bacterial
Humans
In Vitro Techniques
Macrophages ultrastructure
Microscopy, Electron
Mitochondrial ADP, ATP Translocases genetics
Reverse Transcriptase Polymerase Chain Reaction
Vacuoles microbiology
Vacuoles ultrastructure
Chlamydiales pathogenicity
Macrophages microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 71
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 14500518
- Full Text :
- https://doi.org/10.1128/IAI.71.10.5979-5985.2003