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The (4;11)(q21;q23) chromosome translocations in acute leukemias involve the VDJ recombinase.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1992 Nov 01; Vol. 89 (21), pp. 10464-8. - Publication Year :
- 1992
-
Abstract
- Chromosomal region 11q23 is frequently rearranged in acute lymphocytic leukemias (ALLs) and in acute myeloid leukemias (AMLs), mostly in reciprocal exchanges with various translocation partners. The most common of these translocations is t(4;11)(q21;q23). It is present in approximately 10% of ALL patients, most frequently in very young children. We have recently cloned a region of chromosome 11, the ALL-1 locus, found to be rearranged in malignant cells from patients with the t(4;11), t(9;11), t(11;19), t(1;11), t(6;11), t(10;11), and del(11q23) chromosomal abnormalities. Here we report the cloning and characterization of chromosomal breakpoints from leukemic cells with t(4;11) aberrations. The breakpoints cluster in regions of 7-8 kilobases on both chromosomes 4 and 11. The presence of heptamer- and nonamer-like sequences at the sites of breakage suggests that the VDJ recombinase utilized for immunoglobulin gene rearrangement is also directly involved in these translocations. We also show that leukemic cells with t(4;11) express altered RNAs transcribed from the derivative chromosomes 11 and 4.
- Subjects :
- Acute Disease
Base Sequence
Cell Line
Centromere ultrastructure
Chromosome Deletion
DNA, Neoplasm genetics
DNA, Neoplasm isolation & purification
Gene Amplification
Humans
Leukemia, Myeloid enzymology
Molecular Sequence Data
Oligodeoxyribonucleotides
RNA, Neoplasm genetics
RNA, Neoplasm isolation & purification
Receptors, Antigen, T-Cell genetics
Restriction Mapping
Sequence Homology, Nucleic Acid
Telomere ultrastructure
VDJ Recombinases
Chromosomes, Human, Pair 11 ultrastructure
Chromosomes, Human, Pair 4 ultrastructure
DNA Nucleotidyltransferases genetics
Leukemia, Myeloid genetics
Translocation, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 89
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 1438235
- Full Text :
- https://doi.org/10.1073/pnas.89.21.10464