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[Affinity modification of 80S ribosomes from human placenta with mRNA analogs--derivatives of oligouridylates with alkylating groups at the 3'-end].
- Source :
-
Molekuliarnaia biologiia [Mol Biol (Mosk)] 1992 Jul-Aug; Vol. 26 (4), pp. 821-8. - Publication Year :
- 1992
-
Abstract
- 2',3'-O-[4-(N-2-chloroethyl-N-methylamino)]benzylidene derivatives of oligoribonucleotides (Up)5U[32P]pC and (Up)11U[32P]pC were used for affinity labeling of 80S ribosomes from human placenta in the presence of cognate Phe-tRNA(Phe). The derivatives retained the ability of tRNA(Phe)-dependent binding with 80S ribosomes and within the corresponding complexes were cross-linked to 40S subunits (preferentially to 18S rRNA). Sites of the mRNA analogs attachment were identified by blot-hybridization of the modified rRNA with restriction fragments of corresponding rDNA. They are located within positions 1610-1747 and 1748-1865 for both analogs. These fragments are situated at the 3'-end region of 18S rRNA in a highly conserved part of eukaryotic small subunit rRNA secondary structure.
- Subjects :
- Affinity Labels
Alkylating Agents
Blotting, Southern
Cross-Linking Reagents
Female
Humans
Kinetics
Nitrogen Mustard Compounds chemistry
Nucleic Acid Conformation
Pregnancy
Oligoribonucleotides chemistry
Placenta ultrastructure
RNA, Messenger chemistry
Ribosomes chemistry
Uracil Nucleotides chemistry
Subjects
Details
- Language :
- Russian
- ISSN :
- 0026-8984
- Volume :
- 26
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Molekuliarnaia biologiia
- Publication Type :
- Academic Journal
- Accession number :
- 1435775